Built on a sheer rock pinnacle, the Monastery of the Holy Trinity stands as a testament to spiritual devotion. Although references to the monastery date back to 1362, prevailing opinion attributes its construction to the monk Dometios in 1438. The oldest part of the monastery, the current katholikon, was erected between 1456 and 1476, as indicated by the relevant inscription, and is dedicated to the Holy Trinity. In 1741, during the reign of Metropolitan Theofanis of Stagon and under the authority of Abbot Parthenios, the church was adorned with frescoes by the priest Antonios and his brother Nikolaos.

The monastery's katholikon follows the cross-inscribed plan, with a dome supported by two columns. In 1689, a small sacristy was added in the southeast corner, while the wide narthex, with its vaulted ceiling, was constructed the same year and painted in 1692. The rock-hewn chapel of Saint John the Baptist, in the form of a rotunda and adorned with frescoes from 1682, once housed significant treasures, which were unfortunately looted by the Nazi forces during World War II.

Adjacent to the entrance stands the tower of the brizon and the bank, while the cells occupy the opposite side. Behind the katholikon, at the pinnacle of the rock, lies an exterior space. Below, the city of Kalampaka extends to the south, with the lazy Pinei river meandering nearby.

Throughout much of the 20th century, the monastery housed only a handful of monks, numbering no more than 20, and remained uninhabited from 1942 to 1961. In 1972, the Archaeological Service initiated a renovation project.

Traditionally, access to the monastery was challenging, requiring the use of nets or winding ladders. In 1925, 140 steps were constructed, along with a narrow path, to facilitate access. In the 1970s, an aerial transporter (cable car) was installed, further easing visitation.

Visitors are asked to adhere to a modest dress code: men should avoid sleeveless tops and shorts above the knee, while women are encouraged to wear long skirts below the knee when entering the monastery.